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Pipettes with one mark. Pipettes with one mark GOST 29169 laboratory glassware

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INTERSTATE STANDARD

LABORATORY GLASS TABLEWARE

PIPTETTES WITH SINGLE MARK

Official publication

Standardinform

UDC 542.3:006.354

INTERSTATE

Group P66

STANDARD

Laboratory glassware GOST

PIPTETTES with one MARK

Laboratory glassware. One-mark pipettes

(ISO 648-77)

MKS 71.040.20 OKP 43 2133

Date of introduction 01/01/94

1. PURPOSE AND SCOPE OF APPLICATION

This standard covers a range of single mark pipettes for general laboratory use. General requirements- according to GOST 29044.

The requirements of this standard are mandatory.

Additional requirements reflecting the needs of the national economy are given in Appendix 1.

2. LINKS

4. ACCURACY OF VOLUME MEASUREMENT

Two accuracy classes are established:

1st - for higher measurement accuracy;

2nd - for less accurate measurements.

5. RANGE OF CAPACITIES

Pipettes with one mark must be manufactured with a nominal capacity of: 0.5; 1; 2; 5; 10; 20; 25; 50; 100 and 200 ml.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark with a nominal capacity of 10.77 ml.

Pipettes with a nominal capacity of 0.5 ml are manufactured without a reservoir, 1 ml - with and without a reservoir, 2 ml - with a reservoir for the 1st class and with and without a reservoir for the 2nd class.

All other sizes are for pipettes with reservoirs.

Official publication

Reproduction is prohibited

GOST 29169-91 S. 3

7.2. The relationship between the nominal capacity and the permissible error limit of the nominal capacity is given in Appendix A, and the relationship between the permissible error limit of the nominal capacity and the meniscus diameter is given in Appendix B.

8. CONSTRUCTION

8.1. Material

Single mark pipettes shall be made of glass with adequate chemical and thermal resistance and, if possible, free from visible defects and internal tension.

Pipettes with a nominal capacity of 0.5 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and class 2 pipettes with a nominal capacity of 2 ml may have the following drawing. 1 design or may have a reservoir as indicated for larger sizes.

All large pipettes consist of a reservoir with a suction and drain tube. These parts must be straight and aligned.

The shape of the pipettes must correspond to that shown in Fig. 1.

If a safety reservoir is provided, it is approximately spherical in shape and is located between the graduation mark and the top of the pipette.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark in the following designs:

1 - straight (Fig. 2);

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with expansion and spare tank (Fig. 2).

If necessary, pipettes of all sizes can be manufactured with safety reservoirs above the graduation mark.

Note. If necessary, pipettes of other nominal capacities may be manufactured provided that they comply with the essential requirements of this standard.

6. DETERMINATION OF CAPACITY

The capacity of a single-mark pipette is defined as the volume of water, expressed in milliliters, at 20°C, as described below.

The pipette, in a vertical position, should be filled with liquid a few millimeters above the graduation mark, and the drop of the meniscus should then be adjusted to the mark by one of the methods described below:

a) the meniscus is installed so that the plane of the upper edge of the graduation mark is horizontal tangent to the lower point of the meniscus, the observer’s gaze is in the same plane;

b) the meniscus is installed so that the plane of the center of the mark is horizontal tangent to the lower point of the meniscus. The observer's gaze is directed upward towards this plane, while the front and rear parts of the mark should be simultaneously visible, simultaneously converging at the bottom of the meniscus.

Then remove any drops that adhered to the spout tip when the spout tip came into contact with the surface of the glass container.

After this, the liquid must be drained into another, slightly tilted glass vessel so that the drain tip is in contact with the inner wall of the vessel, but without moving them relative to each other during the drain and waiting period.

The pipette is released until the meniscus drops to the drain hole. To ensure that the drainage has finished, wait 3 s before the pipette is removed from the vessel. This applies to pipettes for which there is no set waiting time.

Note. A waiting period (approximately 3 seconds) is set to determine when to drain the liquid. There is no need to strictly adhere to this time. Be sure that the meniscus has reached the outlet end of the pipette before removing the pipette from the receiving vessel.

If a pipette has a waiting time of 15 seconds, this time must be maintained before removing the pipette from the receiving container.

±0.01

±0.02

±0.1

±0.01

±0.02

±0.1

±0.2

Example symbol pipettes used for the needs of the national economy, class 1, class 2, nominal capacity 50 ml:

8.3. Dimensions

The dimensions of the pipettes must correspond to those indicated in the table. 2 and 3.

Dimensions in mm

table 2

Size name	Nominal capacity, ml
Size name
Total length, no more: straight pipettes pipettes with reservoir
Length of suction tube 1), not less
Length of drain pipe 1), not less
Inner diameter at graduation mark 2), no more
Outer diameter of drain pipe 1) (limit deviation ±1 mm)
Tank diameter 1), no more
Straight pipette tube diameter, no more

^Only for pipettes with reservoir.

^ For straight pipettes - maximum internal diameter; for pipettes with a reservoir - the maximum internal diameter of the suction tube.

^ The calculated minimum distance from the graduation mark to the end of the drain hole is 120 mm (see Table 3).

^ For pipettes used for the needs of the national economy.

The outer diameter of the drain tube and the diameter of the straight pipette tube for pipettes used for national household needs are recommended.

Table 3

Meaning

Size name

Distance of the graduation mark from the top of the pipette, not less Distance from the graduation mark to the top of the reservoir, not less Distance from the graduation mark to the drain cone of straight pipettes, not less

Pipette wall thickness, not less: with a reservoir with a nominal capacity of 1 and 2 ml of other pipettes

Safety tank diameter

Distance from the top of the pipette to the bottom of the safety reservoir, no more Distance from the top of the pipette to the top of the safety reservoir, no less

Note. Allowed for pipettes used for the needs of the national economy:

1) the thickness of the expansion wall for pipettes with a reservoir is at least 0.4 mm;

2) the value of the diameter of the safety tank - take it as a size for reference.

GOST 29169-91 S. 5

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that could interfere with the establishment of the meniscus.

The ends of the glass tubes should be fused or polished smooth with a slight bevel outward.

8.5. Storage tank

The main body of the reservoir is cylindrical, with the exception of pipettes with a nominal capacity of 1 and 2 ml, which may have an oval reservoir if required. The two ends of the tank should gradually transition into the suction and drain pipes. To facilitate drainage of the pipette, the connections should be cone-shaped.

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp narrowings at the drain hole.

8.6.2. The end of the drain tip should be treated using one of the methods described below:

a) smoothly ground perpendicular to the axis, slightly beveled outward and melted;

b) smoothly ground perpendicular to the axis and slightly beveled outward;

c) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of fracture during use, but should not cause narrowing, as indicated in and. 8.6.1, or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to descend from the graduation mark to the drain hole.

The drain time is determined with the pipette in a vertical position and the receiving vessel should be slightly tilted so that the tip of the drain tube is in contact with the inner wall of the vessel, but without moving relative to each other.

Thus, the drain time must be within the limits specified in the table. 4.

Table 4

Accuracy class		Drain time, s, for pipettes with nominal capacity,
Accuracy class
1st class without a specific waiting time: no less

1st class with waiting time 15 s: no less

2nd class: no less

The maximum permissible difference between the actual and indicated drain time

^ If the drainage time is indicated on the pipette in accordance with and. 10.2, then the actual and designated time values should not differ by more than the value indicated in the table. 4.

^ For pipettes used for the needs of the national economy.

9. GRADING MARK

The graduation mark must be a clear, uniform, indelible line that completely encircles the tube. A break in the mark not exceeding 10% of the circumference is allowed. The line should be in a plane perpendicular to the axis of the pipette. The thickness of the line should not exceed 0.4 mm, which is provided for by the requirements and. 8.2 GOST 29044.

The dimensions that determine the position of the calibration mark on the pipette are shown in table. 2.

10. LETTERINGS

10.1. All pipettes must be marked with the following markings:

a) a number indicating the nominal capacity and the abbreviation cm 3 or ml to indicate the units in which the pipette is calibrated (see note to clause 3.1);

Note. Where the standard temperature is 27 °C, indicate this value instead of 20 °C;

c) the letter “O” indicating that the pipette is designed to discharge the corresponding nominal capacity;

d) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

e) trademark or name of the manufacturer;

e) the waiting time, if set, in the form “0+15 s”.

10.2. Pipettes of the 1st class intended for official verification or certification should be marked with additional markings, which should preferably be applied to other pipettes of the 1st class, and, if necessary, to the pipettes of the 2nd class:

a) designation of this standard;

b) drain time in seconds.

10.3. Limit of permissible error of nominal capacity in accordance with table. 1 can be applied to all pipettes, for example + ... ml.

11. GRADUATION MARKS, NUMBERS AND INSTRUCTIONS

11.1. All numbers and symbols must be of such size and shape as to be clearly visible under normal conditions of use.

11.2. The graduation mark, numbers and symbols must be clearly visible and indelible.

12. COLOR CODING

If color coding is used, it must be performed in accordance with the requirements of regulatory and technical documentation approved in in the prescribed manner.

APPENDIX A

RELATIONSHIP BETWEEN CAPACITY AND PERMISSIBLE ERROR LIMIT FOR CLASS 1 PIPETTES IN ACCORDANCE WITH THE REQUIREMENTS OF clause 4.4 GOST 29044

ANNEX 1

ADDITIONAL REQUIREMENTS REFLECTING THE NEEDS OF THE NATIONAL ECONOMY

13. Test methods

13.1. The nominal capacity of pipettes should be checked in accordance with the regulatory and technical documentation approved in the prescribed manner.

13.2. The dimensions of pipettes (and. 8.3) should be checked with a universal measuring instrument in accordance with GOST 166 and GOST 427.

13.3. The drainage time of the pipettes should be checked by and. 8.7 of this standard.

14. Packaging, transportation and storage

14.1. Pipettes are packaged in such a way as to ensure their safety during transportation and storage.

14.2. Pipettes packaged by and. 14.1, are placed in transport containers, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

INFORMATION DATA

1. PREPARED AND INTRODUCED by the Elinsky Independent Design and Technology Bureau for the design of glass instruments and apparatus

2. APPROVED AND ENTERED INTO EFFECT by Resolution of the Committee of Standardization and Metrology of the USSR dated December 16, 1991 No. 1952

This standard has been prepared by direct application method. international standard ISO 648-77 “Laboratory glassware. Pipettes with one mark" with additional requirements reflecting the needs of the national economy

Annex 1

Sec. 1, 2, 3, 9; appendices A and B

5. REPUBLICATION. March 2008

GOST 29169-91 (ISO 648-77)

INTERSTATE STANDARD

LABORATORY GLASS TABLEWARE

PIPTETTES WITH SINGLE MARK

Official publication

Standardinform

UDC 542.3:006.354

INTERSTATE

Group P66

STANDARD

Laboratory glassware GOST

PIPTETTES with one MARK

Laboratory glassware. One-mark pipettes

MKS 71.040.20 OKP 43 2133

Date of introduction 01/01/94

1. PURPOSE AND SCOPE OF APPLICATION

This standard covers a range of single mark pipettes for general laboratory use. General requirements - according to GOST 29044.

The requirements of this standard are mandatory.

Additional requirements reflecting the needs of the national economy are given in Appendix 1.

2. LINKS

GOST 29044-91 (ISO 384-78) Laboratory glassware. Principles of arrangement and design of measuring glassware

3. BASIC UNITS OF MEASUREMENT

3.1. Capacity unit

A cubic centimeter (cm 3) is taken as a unit of capacity; the name milliliter (ml) can be used.

Note. The term milliliter (ml) is usually used as a special name for cubic centimeter (cm 3) according to International system units (SI).

3.2. Standard temperature

The standard temperature, i.e. the temperature at which the nominal volume (nominal capacity) is drained, is 20 °C.

Note. When using pipettes in tropical conditions, it is permissible to take the standard water temperature as 27 °C rather than 20 °C, as recommended by GOST 29044.

4. ACCURACY OF VOLUME MEASUREMENT

Two accuracy classes are established:

1st - for higher measurement accuracy;

2nd - for less accurate measurements.

5. RANGE OF CAPACITIES

Pipettes with one mark must be manufactured with a nominal capacity of: 0.5; 1; 2; 5; 10; 20; 25; 50; 100 and 200 ml.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark with a nominal capacity of 10.77 ml.

All other sizes are for pipettes with reservoirs.

Official publication

Reproduction is prohibited

If necessary, pipettes of all sizes can be manufactured with safety reservoirs above the graduation mark.

Note. If necessary, pipettes of other nominal capacities may be manufactured provided that they comply with the essential requirements of this standard.

The capacity of a single-mark pipette is defined as the volume of water, expressed in milliliters, at 20°C, as described below.

a) the meniscus is installed so that the plane of the upper edge of the graduation mark is horizontal tangent to the lower point of the meniscus, the observer’s gaze is in the same plane;

Then remove any drops that adhered to the spout tip when the spout tip came into contact with the surface of the glass container.

If a pipette has a waiting time of 15 seconds, this time must be maintained before removing the pipette from the receiving container.

Note. Class 1 pipettes must be:

a) without a specific waiting time, when this time is approximately 3 s;

b) with a waiting time of 15 s.

Class 2 pipettes must have no specified waiting time as defined in subparagraph a.

6. DETERMINATION OF CAPACITY

Permissible error limit

Nominal capacity

±0.01

±0.02

±0.1

±0.01

±0.02

±0.1

±0.2

8. CONSTRUCTION

8.1. Material

Single mark pipettes should be made of glass with adequate chemical and thermal resistance and, if possible, free from visible defects and internal stress.

Pipettes with a nominal capacity of 0.5 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and class 2 pipettes with a nominal capacity of 2 ml may have the following drawing. 1 design or may have a reservoir as indicated for larger sizes.

All large pipettes consist of a reservoir with a suction and drain tube. These parts must be straight and aligned.

The shape of the pipettes must correspond to that shown in Fig. 1.

If a safety reservoir is provided, it is approximately spherical in shape and is located between the graduation mark and the top of the pipette.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark in the following designs:

1 - straight (Fig. 2);

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with expansion and spare tank (Fig. 2).

Execution 1 Execution 1a Execution 2 Execution 2a

An example of a symbol for a pipette used for the needs of the national economy, class 1, class 2, nominal capacity 50 ml:

Pipette 1-2-50 GOST 29169-91

8.3. Dimensions

The dimensions of the pipettes must correspond to those indicated in the table. 2 and 3.

Dimensions in mm

table 2

Size name	Nominal capacity, ml
Size name
Total length, no more: straight pipettes pipettes with reservoir
Length of suction tube 1), not less
Length of drain pipe 1), not less
Inner diameter at graduation mark 2), no more
Outer diameter of drain pipe 1) (limit deviation ±1 mm)
Tank diameter 1), no more
Straight pipette tube diameter, no more

^Only for pipettes with reservoir.

^ For straight pipettes - maximum internal diameter; for pipettes with a reservoir - the maximum internal diameter of the suction tube.

^ The calculated minimum distance from the graduation mark to the end of the drain hole is 120 mm (see Table 3).

^ For pipettes used for the needs of the national economy.

The outer diameter of the drain tube and the diameter of the straight pipette tube for pipettes used for national household needs are recommended.

Table 3

Size name

Meaning

Pipette wall thickness, not less: with a reservoir with a nominal capacity of 1 and 2 ml of other pipettes

Safety tank diameter

Distance from the top of the pipette to the bottom of the safety reservoir, no more Distance from the top of the pipette to the top of the safety reservoir, no less

Note. Allowed for pipettes used for the needs of the national economy:

1) the thickness of the expansion wall for pipettes with a reservoir is at least 0.4 mm;

2) the value of the diameter of the safety tank - take it as a size for reference.

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that could interfere with the establishment of the meniscus.

The ends of the glass tubes should be fused or polished smooth with a slight bevel outward.

8.5. Storage tank

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp narrowings at the drain hole.

8.6.2. The end of the drain tip should be treated using one of the methods described below:

a) smoothly ground perpendicular to the axis, slightly beveled outward and melted;

b) smoothly ground perpendicular to the axis and slightly beveled outward;

c) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of fracture during use, but should not cause narrowing, as indicated in and. 8.6.1, or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to descend from the graduation mark to the drain hole.

Thus, the drain time must be within the limits specified in the table. 4.

Table 4

Accuracy class		Drain time, s, for pipettes with nominal capacity,
Accuracy class
1st class without a specific waiting time: no less

1st class with waiting time 15 s: no less

2nd class: no less

The maximum permissible difference between the actual and indicated drain time

^ If the drainage time is indicated on the pipette in accordance with and. 10.2, then the actual and designated time values should not differ by more than the value indicated in the table. 4.

^ For pipettes used for the needs of the national economy.

9. GRADING MARK

The dimensions that determine the position of the calibration mark on the pipette are shown in table. 2.

10. LETTERINGS

10.1. All pipettes must be marked with the following markings:

a) a number indicating the nominal capacity and the abbreviation cm 3 or ml to indicate the units in which the pipette is calibrated (see note to clause 3.1);

Note. Where the standard temperature is 27 °C, indicate this value instead of 20 °C;

c) the letter “O” indicating that the pipette is designed to discharge the corresponding nominal capacity;

d) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

e) trademark or name of the manufacturer;

e) the waiting time, if set, in the form “0+15 s”.

a) designation of this standard;

b) drain time in seconds.

10.3. Limit of permissible error of nominal capacity in accordance with table. 1 can be applied to all pipettes, for example ±... ml.

11. GRADUATION MARKS, NUMBERS AND INSTRUCTIONS

11.1. All numbers and symbols must be of such size and shape as to be clearly visible under normal conditions of use.

11.2. The graduation mark, numbers and symbols must be clearly visible and indelible.

12. COLOR CODING

If color coding is used, it must be performed in accordance with the requirements of regulatory and technical documentation approved in the prescribed manner.

APPENDIX A

RELATIONSHIP BETWEEN CAPACITY AND PERMISSIBLE ERROR LIMIT FOR CLASS 1 PIPETTES IN ACCORDANCE WITH THE REQUIREMENTS OF clause 4.4 GOST 29044

APPENDIX B

RELATIONSHIP BETWEEN THE LIMITS OF PERMISSIBLE ERROR AND THE DIAMETER OF THE MENISCUS FOR PIPETTES 1ST CLASS IN ACCORDANCE WITH THE REQUIREMENTS OF clause 4.7 GOST 29044

Limit of permissible error for Hgo class, ± µl

Meniscus diameter, mm

ANNEX 1

ADDITIONAL REQUIREMENTS REFLECTING THE NEEDS OF THE NATIONAL ECONOMY

13. Test methods

13.1. The nominal capacity of pipettes should be checked in accordance with the regulatory and technical documentation approved in the prescribed manner.

13.2. The dimensions of pipettes (i. 8.3) should be checked with a universal measuring instrument in accordance with GOST 166 and GOST 427.

13.3. The drainage time of the pipettes should be checked by and. 8.7 of this standard.

14. Packaging, transportation and storage

14.1. Pipettes are packaged in such a way as to ensure their safety during transportation and storage.

14.2. Pipettes packaged by and. 14.1, are placed in transport containers, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

INFORMATION DATA

1. PREPARED AND INTRODUCED by the Elinsky Independent Design and Technology Bureau for the design of glass instruments and apparatus

2. APPROVED AND ENTERED INTO EFFECT by Resolution of the Committee of Standardization and Metrology of the USSR dated December 16, 1991 No. 1952

This standard has been prepared by direct application of the international standard ISO 648-77 “Laboratory glassware. Pipettes with one mark" with additional requirements reflecting the needs of the national economy

3. INSTEAD GOST 20292-74 regarding pipettes with one mark

4. REFERENCE REGULATIVE AND TECHNICAL DOCUMENTS

5. REPUBLICATION. March 2008

UDC 542.3:006.3S4 Group 1166

INTERSTATE STANDARD

Laboratory glassware PIPTES WITH SINGLE MARK

Laboratory glassware. One-mark pipettes

MKS 71.040.20 OKP 43 2133

Date of introduction 01/01/94

I. PURPOSE AND SCOPE OF APPLICATION

This standard covers a range of single mark pipettes for general laboratory use. General requirements - according to GOST 29044.

The requirements of this standard are mandatory.

Shown in Appendix 1.

If necessary, pipettes of all sizes can be manufactured with safety reservoirs above the graduation mark.

Note. If necessary, pipettes of other nominal capacities may be manufactured provided that they comply with the essential requirements of this standard.

6. DETERMINATION OF CAPACITY

The capacity of a single-mark pipette is defined as the volume of water expressed in milliliters. temperature 20°C, as described below.

a) the meniscus is installed like this. so that the plane of the upper edge of the graduation mark is horizontal tangent to the lower point of the meniscus, the observer’s gaze is in the same plane;

b) the meniscus is installed like this. so that the plane of the center of the mark is horizontal tangent to the lower point of the meniscus. The observer's gaze is directed upward towards this plane, while the front and rear parts of the mark should be simultaneously visible, simultaneously converging at the bottom of the meniscus.

Then remove any drops that adhered to the spout tip when the spout tip came into contact with the surface of the glass container.

The pipette is released until then. until the meniscus descends to the drain hole. To ensure that the drainage has finished, wait 3 s before the pipette is removed from the vessel. This applies to pipettes for which there is no set waiting time.

Note: The waiting period (approximately 3 seconds) is set to determine when to drain the fluid. There is no need to strictly adhere to this time. Be sure that the meniscus has reached the outlet end of the pipette before removing the pipette from the receiving vessel.

If a pipette has a waiting time of 15 seconds, this time must be waited before removing the pipette from the receiving vessel.

Primschan and s. Class I pipettes must be:

a) without a specific waiting time, when this time is approximately 3 s;

b) with a waiting time of 15 s.

Class 2 pipettes must have no specified waiting time as defined in subparagraph a.

7. VOLUME PERMISSIBLE ERROR LIMITS

7.1. The limits of permissible error of the nominal capacity should not exceed the values specified in table. 1.

Table I

11 For pipettes used for the needs of the national economy.

7.2. The relationship between the nominal capacity and the maximum permissible error of the nominal capacity is given in Appendix A, and the relationship between the maximum permissible error of the nominal capacity and the diameter of the meniscus is given in Appendix B.

8. CONSTRUCTION

8.1. Material

Single mark pipettes should be made of glass with adequate chemical and thermal resistance and, if possible, free from visible defects and internal stress.

Pipettes with a nominal capacity of 0.5 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and class 2 pipettes with a nominal capacity of 2 ml may have the following drawing. I design or may have a reservoir as indicated for larger sizes.

All large pipettes consist of a reservoir with a suction and drain tube. These parts must be straight and aligned.

The shape of the pipettes must correspond to that shown in Fig. 1.

If a safety reservoir is provided, it is approximately spherical in shape and is located between the graduation mark and the top of the pipette.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark in the following designs:

1 - straight (Fig. 2);

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with expansion and spare tank (Fig. 2).

Execution /

Execution 1a Execution 2

Version 2a

An example of a symbol for a pipette used for the needs of the national economy, class 1. 2, nominal capacity 50 ml:

8.3. Dimensions

The dimensions of the pipettes must correspond to those indicated in the table. 2 and 3.

Tabliya 2

Dimensions in mm

Rusher name	Nomiih11.na!1 capacity, ml
Rusher name
Total length, no more: straight lip pipettes with reservoir
The length of the suction tube is not less than
Drain tube length >, not less
Inner diameter at graduation mark 2 *, no more
Outer diameter of drain pipe 1" (maximum deviation ±1 mm)
Tank diameter 11, no more
Straight pipette tube diameter, no more

""Only for pipettes with reservoir.

For straight pipettes - the maximum internal diameter: for pipettes with a reservoir - the maximum internal diameter of the suction tube.

3> The estimated minimum distance from the graduation mark to the drain hole head is 120 mm (see Table 3).

41 For pipettes used for the needs of the national economy.

The outer diameter of the drain tube and the diameter of the straight pipette tube for pipettes, using

for the needs of the national economy are recommended.	Table 3
Size name	Meaning
Distance of the calibration mark from the top of the pipette, not less
Distance from the calibration mark to the top of the tank, not less
Distance from the graduation mark to the outlet cone of straight pipettes.

Pipette wall thickness, not less:
with a reservoir with a nominal capacity of 1 and 2 ml
other pipettes
Safety tank diameter
Distance from the top of the pipette to the bottom of the safety tank, no more
Distance from the top of the pipette to the top of the safety reservoir.

Note: Allowed for pipettes used for the needs of the national economy:

1) the thickness of the expansion wall for pipettes with a reservoir is at least 0.4 mm:

2) the value of the diameter of the safety tank - take it as a size for reference.

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that could interfere with the establishment of the meniscus.

The ends of the glass tubes should be fused or polished smooth with a slight bevel outward.

8.5. Storage tank

The main part of the reservoir is cylindrical, with the exception of pipettes with a nominal capacity of 1 and 2 ml. which may have, if necessary, a disgraced reservoir. The two ends of the tank should gradually transition into the suction and drain pipes. To facilitate drainage of the pipette, the connections should be cone-shaped.

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp narrowings at the drain hole.

8.6.2. The end of the drain tip should be treated using one of the methods described below:

a) smoothly ground perpendicular to the axis, slightly beveled outward and plated;

b) smoothly ground perpendicular to the axis and slightly beveled outward;

c) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of fracture during use, but should not cause contraction, as specified in paragraph 8.6.1, or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to descend from the graduation mark to the drain hole.

Thus, the drain time must be within the limits specified in the table. 4.

Table 4

Youth class		Time elnnl. With. for pinsyuk with nominal capacity.
Youth class
1st class without a specific waiting time: no less

1st class with waiting time 15 s: no less

2nd class: no less

Maximum permissible difference between actual and indicated drain time 1 *

If the draining time is indicated on the pipette in accordance with clause 10.2, the actual and indicated time values should not differ by more than the value indicated in the table. 4.

2> For pipettes used for the needs of the national economy.

9. GRADING MARK

The graduation mark must be a clear, uniform, indelible line that completely encircles the tube. A break in the mark not exceeding 10% of the circumference is allowed. The line should be in a plane perpendicular to the axis of the pipette. The line thickness should not exceed 0.4 mm. which is provided for by the requirements of clause 8.2 of GOST 29044.

The dimensions that determine the position of the calibration mark on the pipette are shown in table. 2.

10. LETTERINGS

10.1. All pipettes must be marked with the following markings:

a) a number indicating the nominal capacity and the abbreviation cm 3 or ml to indicate the units in which the pipette is calibrated (see note to clause 3.1);

Pri m scha n i s. There. where the standard temperature is 27 "C, indicate this value instead of 20" C;

c) the letter "O". indicating that the pipette is intended to discharge the corresponding nominal capacity:

d) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

e) trademark or name of the manufacturer:

e) the waiting time, if set, in the form “0+15 s*.

10.2. For class 1 pipettes intended for official verification or certification. Additional markings should be applied, which should preferably be applied to other class 1 pipettes, and, if necessary, to class 2 pipettes:

a) designation of this standard;

b) drain time in seconds.

10.3. Limit of permissible error of nominal capacity in accordance with table. 1 can be applied to all pipettes, for example ±. . . ml.

11. GRADUATION MARKS, NUMBERS AND INSTRUCTIONS

11.1. All numbers and symbols must be of such size and shape as to be clearly visible under normal conditions of use.

P.2. The graduation mark, numbers and symbols must be clearly visible and indelible.

12. COLOR CODING

If color coding is used, it must be performed in accordance with the requirements of regulatory and technical documentation approved in the prescribed manner.

APPENDIX A

RELATIONSHIP BETWEEN CAPACITY AND PERMISSIBLE ERROR LIMIT FOR CLASS 1 PIPETTES IN ACCORDANCE WITH THE REQUIREMENTS OF clause 4.4 GOST 29044

APPENDIX B

RELATIONSHIP BETWEEN THE LIMITS OF PERMISSIBLE ERROR AND THE DIAMETER OF THE MENISCUS FOR CLASS 1 PIPTES IN ACCORDANCE WITH THE REQUIREMENTS a. 4.7 GOST 29044

4.5 5.5 6 7,5 $5

Divngtr meniscus, nor

APPLICATION /

ADDITIONAL REQUIREMENTS REFLECTING THE NEEDS OF THE NATIONAL ECONOMY

13. Test tables

13.1. The nominal capacity of pipettes must be checked in accordance with the regulatory and technical documentation approved in the prescribed manner.

13.2. The dimensions of the pipettes (clause S.3) should be checked with a universal measuring instrument in accordance with GOST 166 and GOST 427.

13.3. The drainage time of the pipettes should be checked according to clause 8.7 of this standard.

14. Packaging, transportation and storage

14.2. Pipettes packaged according to clause 14.1. placed in transport containers, where they must be protected from movement and damage.

INFORMATION DATA

1. PREPARED AND INTRODUCED by the Klin Independent Design and Technology Bureau for the design of glass instruments and apparatus

2. APPROVED AND ENTERED INTO EFFECT by Resolution of the Committee of Standardization and Metrology of the USSR dated December 16, 1991 No. 1952

This standard has been prepared by direct application of the international standard ISO 648-77 *Laboratory glass vessels. Pipettes with one mark" with additional requirements reflecting the needs of the national economy

APPENDIX I

ADDITIONAL REQUIREMENTS REFLECTING THE NEEDS OF THE NATIONAL ECONOMY

13. Test methods

13.1. The nominal capacity of pipettes should be checked in accordance with the regulatory and technical documentation approved in the prescribed manner.

13.2. The dimensions of the pipettes (clause 8.3) should be checked with a universal measuring instrument in accordance with GOST 166 and GOST 427.

13.3. The draining time of the pipettes should be checked according to clause S.7 of this standard.

14. Packaging, transportation and storage

14.1. Pipettes are packaged in such a way as to ensure their safety during transportation and storage.

14.2. Pipettes packaged according to clause 14.1 are placed in transport containers, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

GOST 29169-91
(ISO 648-77)

Group P66

INTERSTATE STANDARD

Laboratory glassware

PIPTETTES WITH SINGLE MARK

Laboratory glassware. One-mark pipettes

Date of introduction 1994-01-01

INFORMATION DATA

1. PREPARED AND INTRODUCED by the Klin Independent Design and Technology Bureau for the design of glass instruments and apparatus

2. APPROVED AND ENTERED INTO EFFECT by Resolution of the Committee of Standardization and Metrology of the USSR dated December 16, 1991 N 1952

This standard has been prepared by direct application of the international standard ISO 648-77 "Laboratory glassware. Pipettes with one mark" with additional requirements reflecting the needs of the national economy

3. INSTEAD GOST 20292-74 regarding pipettes with one mark

4. REFERENCE REGULATIVE AND TECHNICAL DOCUMENTS

Number of section, application in which the link is provided	Designation of the corresponding international standard	Designation of the domestic technical documentation to which the link is given
Annex 1
Annex 1
Section 1, 2, 3, 9; appendices A and B	ISO 384-78

5. REISSUE

1. PURPOSE AND SCOPE OF APPLICATION

This standard covers a range of single mark pipettes for general laboratory use. General requirements - according to GOST 29044.

The requirements of this standard are mandatory.

Additional requirements reflecting the needs of the national economy are given in Appendix 1.

3. BASIC UNITS OF MEASUREMENT

3.1. Capacity unit

The unit of capacity is taken to be a cubic centimeter (cm); the name milliliter (ml) can be used.

Note. The term milliliter (ml) is usually used as a special name for cubic centimeter (cm) according to the International System of Units (SI).

3.2. Standard temperature

Standard temperature, i.e. the temperature at which the nominal volume (nominal capacity) is drained is 20 °C.

Note. When using pipettes in tropical conditions, it is allowed to take 27 °C rather than 20 °C as the standard water temperature, as recommended by GOST 29044.

4. ACCURACY OF VOLUME MEASUREMENT

Two accuracy classes are established:

1st - for higher measurement accuracy;

2nd - for less precise measurements.

5. RANGE OF CAPACITIES

Pipettes with one mark must be manufactured with a nominal capacity of: 0.5; 1; 2; 5; 10; 20; 25; 50; 100 and 200 ml.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark with a nominal capacity of 10.77 ml.

All other sizes are for pipettes with reservoirs.

If necessary, pipettes of all sizes can be manufactured with safety reservoirs above the graduation mark.

Note. If necessary, pipettes of other nominal capacities may be manufactured provided that they comply with the essential requirements of this standard.

6. DETERMINATION OF CAPACITY

The capacity of a single-mark pipette is defined as the volume of water, expressed in milliliters, at 20°C, as described below.

A) the meniscus is installed so that the plane of the upper edge of the graduation mark is horizontal tangent to the lower point of the meniscus, the observer’s gaze is in the same plane;

B) the meniscus is installed so that the plane of the center of the mark is horizontal tangent to the lower point of the meniscus. The observer's gaze is directed upward towards this plane, while the front and rear parts of the mark should be simultaneously visible, simultaneously converging at the bottom of the meniscus.

Then remove any drops that adhered to the spout tip when the spout tip came into contact with the surface of the glass container.

If a pipette has a waiting time of 15 seconds, this time must be maintained before removing the pipette from the receiving container.

Note. 1st class pipettes must be:

A) without a specific waiting time, when this time is approximately 3 s;

B) with a waiting time of 15 s.

Class 2 pipettes must have no specified waiting time as defined in subparagraph a.

7. VOLUME PERMISSIBLE ERROR LIMITS

7.1. The limits of permissible error of the nominal capacity should not exceed the values specified in Table 1.

Table 1

Nominal capacity	Permissible error limit
	1st class	2nd class

* For pipettes used for the needs of the national economy.

8. CONSTRUCTION

8.1. Material

Single mark pipettes should be made of glass with adequate chemical and thermal resistance and, if possible, free from visible defects and internal stress.

8.2. Form

Pipettes with a nominal capacity of 0.5 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and class 2 pipettes with a nominal capacity of 2 ml may have the design shown in Figure 1 or may have a reservoir as indicated for larger sizes.

All large pipettes consist of a reservoir with a suction and drain tube. These parts must be straight and aligned.

The shape of the pipettes must correspond to that shown in Figure 1.

If a safety reservoir is provided, it is approximately spherical in shape and is located between the graduation mark and the top of the pipette.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark in the following designs:

1 - straight (Fig. 2);

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with expansion and spare tank (Fig. 2).

An example of a symbol for a pipette used for the needs of the national economy, class 1, class 2, nominal capacity 50 ml:

Pipette 1-2-50 GOST 29169-91

8.3. Dimensions

The dimensions of the pipettes must correspond to those indicated in Tables 2 and 3.

table 2

Dimensions in mm

Size name	Nominal capacity, ml

Total length, no more:
straight pipettes
pipettes with reservoir		550; 560****
Length of suction tube*, not less
Length of drain pipe*, not less
Inner diameter at graduation mark**, no more
Outer diameter of drain pipe* (limit deviation ±1 mm)
Tank diameter*, no more
Straight pipette tube diameter, no more

*Only for pipettes with reservoir.

** For straight pipettes - maximum internal diameter; for pipettes with a reservoir - the maximum internal diameter of the suction tube.

*** The estimated minimum distance from the graduation mark to the end of the drain hole is 120 mm (see Table 3).

**** For pipettes used for the needs of the national economy.

Table 3

Size name	Meaning
Distance of the calibration mark from the top of the pipette, not less
Distance from the calibration mark to the top of the tank, not less
Distance from the calibration mark to the drain cone of straight pipettes, not less
Pipette wall thickness, not less:
with a reservoir with a nominal capacity of 1 and 2 ml
other pipettes
Safety tank diameter
Distance from the top of the pipette to the bottom of the safety tank, no more
Distance from the top of the pipette to the top of the safety reservoir, not less

Note. Allowed for pipettes used for the needs of the national economy:

1) the thickness of the expansion wall for pipettes with a reservoir is at least 0.4 mm;

2) the value of the diameter of the safety tank - take it as a size for reference.

The outer diameter of the drain tube and the diameter of the straight pipette tube for pipettes used for national household needs are recommended.

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that could interfere with the establishment of the meniscus.

The ends of the glass tubes should be fused or polished smooth with a slight bevel outward.

8.5. Storage tank

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp narrowings at the drain hole.

8.6.2. The end of the drain tip should be treated using one of the methods described below:

A) smoothly ground perpendicular to the axis, slightly beveled outward and melted;

B) smoothly ground perpendicular to the axis and slightly beveled outward;

C) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of fracture during use, but should not cause contraction, as specified in paragraph 8.6.1, or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to descend from the graduation mark to the drain hole.

Thus, the drain time should be within the limits specified in Table 4.

Table 4

Accuracy class	Drain time, s, for pipettes with nominal capacity, ml

1st class without specific waiting time:


1st class with waiting time 15 s:
no less
no more
2nd class:


Maximum permissible difference between actual and indicated drain time*

* If the draining time is indicated on the pipette in accordance with clause 10.2, then the actual and indicated time values should not differ by more than the value indicated in Table 4.

** For pipettes used for the needs of the national economy.

9. GRADING MARK

The graduation mark must be a clear, uniform, indelible line that completely encircles the tube. It is allowed to break the mark not exceeding 10% of the circumference. The line should be in a plane perpendicular to the axis of the pipette. The line thickness should not exceed 0.4 mm, which is provided for by the requirements of clause 8.2 of GOST 29044.

The dimensions that determine the position of the calibration mark on the pipette are shown in Table 2.

10. LETTERINGS

10.1. All pipettes must be marked with the following markings:

A) a number indicating the nominal capacity and the abbreviation cm or ml to indicate the units in which the pipette is calibrated (see note to clause 3.1);

Note. Where the standard temperature is 27 °C, indicate this value instead of 20 °C;

B) the letter "O", indicating that the pipette is intended to discharge the corresponding nominal capacity;

D) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

D) trademark or name of the manufacturer;

E) the waiting time, if set, in the form "0+15 s".

A) designation of this standard;

B) drain time in seconds.

10.3. The maximum permissible error of the nominal capacity in accordance with Table 1 can be applied to all pipettes, for example, ±. . . ml.

11. GRADUATION MARKS, NUMBERS AND INSTRUCTIONS

11.1. All numbers and symbols must be of such size and shape as to be clearly visible under normal conditions of use.

11.2. The graduation mark, numbers and symbols must be clearly visible and indelible.

12. COLOR CODING

If color coding is used, it must be performed in accordance with the requirements of regulatory and technical documentation approved in the prescribed manner.

APPENDIX A

RELATIONSHIP BETWEEN CAPACITY AND PERMISSIBLE ERROR LIMIT FOR CLASS 1 PIPETTES IN ACCORDANCE WITH THE REQUIREMENTS OF clause 4.4 GOST 29044

14.1. Pipettes are packaged in such a way as to ensure their safety during transportation and storage.

14.2. Pipettes packaged according to clause 14.1 are placed in transport containers, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

The text of the document is verified according to:
official publication
Laboratory glassware and equipment.
Pipettes. Burettes: Sat. GOST. -
M.: IPK Standards Publishing House, 2003